Journal: American Journal of Infection Control

Article Title: Physiologic and other effects and compliance with long-term respirator use among medical intensive care unit nurses

doi: 10.1016/j.ajic.2013.02.017

Figure Lengend Snippet: Impact of long-term respirator usage on physiologic measures and subjective symptoms

Article Snippet: CO 2 and O 2 were measured using a SenTec CO 2 and O 2 saturation sensor (SenTec AG, Therwil, Switzerland).

Techniques:

Journal: American Journal of Infection Control

Article Title: Physiologic and other effects and compliance with long-term respirator use among medical intensive care unit nurses

doi: 10.1016/j.ajic.2013.02.017

Figure Lengend Snippet: Impact of long-term respirator usage on physiologic measures and subjective symptoms when comparing an N95 alone versus an N95 with mask overlay

Article Snippet: CO 2 and O 2 were measured using a SenTec CO 2 and O 2 saturation sensor (SenTec AG, Therwil, Switzerland).

Techniques:

Journal: Proceedings of the National Academy of Sciences of the United States of America

Article Title: Large enrichments in fatty acid 2 H/ 1 H ratios distinguish respiration from aerobic fermentation in yeast Saccharomyces cerevisiae

doi: 10.1073/pnas.2310771121

Figure Lengend Snippet: S. cerevisiae ( A ) lipid/water 2 H-fractionation and ( B ) biomass/substrate 13 C-fractionation for chemostat growth on fermentable sugar and nonfermentable glycerol substrates at different growth rates. Strain EXF-4126 grown with 2% glycerol (blue), 2% glucose (brown); strain FY4 grown with 0.75% glucose (orange), 0.75% galactose (yellow). Panel A bars, abundance weighted average 2 ε fattyacid/water of n = 3 replicate sampling timepoints; error bars are SD. Palmitic ( n -C 16:0 ) and palmitoleic ( n -C 16:1 ) acid 2 ε fattyacid/water indicated by filled and open circles, respectively (circle size, relative abundance of 16 carbon fatty acids). Panel B bars are biomass/substrate 13 C-fractionation, mean of 3 to 6 replicate sampling timepoints; error bars are SD of 3 to 6 timepoint replicates. See details in SI Appendix , Figs. S2 and S3 and Datasets S1–S3 .

Article Snippet: Chemostats consisted of autoclave-sterilized 2 L bioreactors (Chemglass Inc.,) at ~1 L working volumes at 30 °C, 400 rpm, aerated with filtered house air, and equipped with a pH/Temp/ORP InPro sensor (3253i Mettler-Toledo LLC, Columbus, OH), optical O 2 sensors for strain EXF-4126 only (6860i Mettler-Toledo LLC, Columbus, OH), and a data-logging scale to continuously monitor conditions.

Techniques: Fractionation, Sampling

Journal: Proceedings of the National Academy of Sciences of the United States of America

Article Title: Large enrichments in fatty acid 2 H/ 1 H ratios distinguish respiration from aerobic fermentation in yeast Saccharomyces cerevisiae

doi: 10.1073/pnas.2310771121

Figure Lengend Snippet: NADP + -reducing (NADPH-producing) enzyme activities in glycerol-respiring (blue circles) and glucose-fermenting (brown squares) chemostats of strain EXF-4126. ( A – D ) Enzyme activities of 4 to 11 individual sample replicates (colored dots) and their averages (circles and squares). Measured IDH activity for glucose-grown cells is assumed to represent mIDH as the cytosolic form is glucose repressed . ( E ) Total NAPDH production rates and 1 SD propagated errors, assuming comparable total protein extract efficiencies across enzyme assays. All linear regressions are based on averages with adjusted R 2 . See Datasets S1–S4 for details.

Article Snippet: Chemostats consisted of autoclave-sterilized 2 L bioreactors (Chemglass Inc.,) at ~1 L working volumes at 30 °C, 400 rpm, aerated with filtered house air, and equipped with a pH/Temp/ORP InPro sensor (3253i Mettler-Toledo LLC, Columbus, OH), optical O 2 sensors for strain EXF-4126 only (6860i Mettler-Toledo LLC, Columbus, OH), and a data-logging scale to continuously monitor conditions.

Techniques: Activity Assay